Thesis-2004-Barrett.pdf (12.1 MB)
Polymers in microfluidics
thesisposted on 2015-01-20, 09:59 authored by Louise M. Barrett
There is great interest in miniaturized analytical systems for life science research, the clinical environment, drug discovery, biotechnology, quality control, and environmental monitoring and numerous articles have been written which predict the success of microfluidic based systems. It was demonstrated m this work that a microfluidic flow system could be quickly and easily manufactured in a research lab environment without the need for clean room facilities. The microfluidic device was created using polymethyhnethacrylate, a CO2 laser and an standard oven. The device was designed, manufactured and ready for use within three hours. This work also investigated a chemiluminescent system which was intended for use in protease assays in the microfluidic device. This work also focused on the use of photoinitiated polymer monoliths, with immobilized tannic acid, as protein preconcentrators. The function of the monolithic devices was demonstrated by pumping low concentration solutions of BSA BODIPY® FL through the monolith. Both loading and elution were done using pressure. It was shown that BSA could be concentrated on and successfully eluted from the monolith. The elution volume for a 125 nI monolith was found to be 4 Ill. Therefore an injection of a 60 III sample of 1 x 10-'\1 BSA BODIPY ® FL gave rise to a concentration factor of 15. The pH optimum for the binding of BSA BODIPY ® FL was found to be pH 8.0 and the loading capacity of the tannic acid monolith was found to be 0.6 mg_ml-1
Publisher© Louise M. Barrett
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesA Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.
EThOS Persistent IDuk.bl.ethos.413588