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Superheated water as a mobile phase

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thesis
posted on 22.05.2018 by Robert J. Burgess
This project has focused on the applicability of using superheated water as an alternative eluent for use in reversed phase high performance liquid chromatography (RP-HPLC). A system based on instrumentation from gas chromatography (GC), HPLC, and supercritical fluid chromatography (SFC) was utilised with some success. Of particular importance was the stability of the column stationary phases commonly employed in RP-HPLC. The most popular phases based on alkyl bonded silica proved unsuitable due to excessive dissolution of the base silica at high temperatures. Nevertheless, two other phases—one a poly(styrene-divinylbenzene) material and the other a porous graphitic carbon—proved stable to temperatures as high as 240°C. At such temperatures compound stability was good, except for a notable case with nitrobenzene. The mechanism of elution remained reversed phase mode throughout, with polar solutes being eluted before non-polar ones. [Continues.]

History

School

  • Science

Department

  • Chemistry

Publisher

© Robert John Burgess

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

1999

Notes

A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.

Language

en

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