Thesis-1986-Montenegro.pdf (2.7 MB)

Studies of column packings for polymer separations by size exclusion and interactive chromatography

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thesis
posted on 22.02.2013, 14:28 by Ana M.C. Montenegro
The chromatographic behaviour of a new column packing for high performance size exclusion chromatography based on crosslinked polyacrylamide particles was studied. Experimental retention data for poly(ethylene glycol)and poly(ethylene oxide) standards in water and water-methanol mixtures showed that the separation mechanism is size exclusion, since a universal calibration plot based on hydrodynamic volume was obtained. For polysaccharide standards in water and water-methanol (80/20), the universal calibration was valid for molecular weights above 4 * 10~. Below this value, secondary mechanisms appear to be taking part in the separation since water-methanol (80/20) is a poor solvent for polysaccharides. Crosslinked polyacrylamide packings showed an interactive behaviour with tetrahydrofuran and dimethylformamide as eluents with polystyrene and poly( ethylene glycol )/poly(ethylene oxide) standards and the universal calibration was not valid....

History

School

  • Science

Department

  • Chemistry

Publisher

© Ana M.C. Montenegro

Publication date

1986

Notes

A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.

EThOS Persistent ID

uk.bl.ethos.374470

Language

en

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